Kinetic Properties of Purified β-Mannanase from Penicillium italicum
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چکیده
منابع مشابه
extraction and acetylation of purified trypsin from bovin pancreas and study of some its physico-chemical properties
آنزیم تریپسین در شرایط قلیایی ناپایدار می باشد .و فعالیت پروتئولیتیکی تریپسین منجربه خود هضمی آن در جایگاههای خاصی می گردد. بنابر این آنزیمی با ناپایداری بالا محسوب میگردد. در سالهای اخیر موفق شدند که با ایجاد تغیرات شیمیایی با اضافه کردن فلزات خاص ، کلسیم و یا عمل استیلاسیون منجر به افزایش پایداری آنزیم تریپسین گردند. مطالعات در حال حاضر نشان می دهد که تریپسین استیله شده فعالیت آنزیمی خود را ...
15 صفحه اولBiochemical Characterization of an Extracellular β-Glucosidase from the Fungus, Penicillium italicum, Isolated from Rotten Citrus Peel
A β-glucosidase from Penicillium italicum was purified with a specific activity of 61.8 U/mg, using a chromatography system. The native form of the enzyme was an 88.5-kDa tetramer with a molecular mass of 354 kDa. Optimum activity was observed at pH 4.5 and 60℃, and the half-lives were 1,737, 330, 34, and 1 hr at 50, 55, 60, and 65℃, respectively. Its activity was inhibited by 47% by 5 mM Ni(2+...
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In this study, partially purified K+-H+ ATPase from frog gastric mucosa were obtained by using differential and density gradient centrifugation. Optimum activity of K+-H+ ATPase (Vmax), Michealis-Menten constant (Km) and Hill coefficient (h) were found as 83.3 mumol Pi.mg prot-1.h-1, 95.2 microM and 0.91, respectively. Enzyme preparations were more stable in glycerol solutions stored at -40 deg...
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Studies on the antimicrobial properties of light have considerably increased due in part to the development of resistance to actual control methods. This study investigates the potential of light-emitting diodes (LED) blue light for controlling Penicillium digitatum and Penicillium italicum. These fungi are the most devastating postharvest pathogens of citrus fruit and cause important losses du...
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Sterol 14α-demethylases from Cytochrome P450 family (CYP51s) are essential enzymes in sterol biosynthesis and well-known as the target of antifungal drugs. The 3D structure of CYP51A from Penicillium italicum (PiCYP51A) was constructed through homology modeling based on the crystal structure of human CYP51A (PDB: 3LD6). Molecular dynamics (MD) simulation was operated to relax the initial model ...
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ژورنال
عنوان ژورنال: British Microbiology Research Journal
سال: 2014
ISSN: 2231-0886
DOI: 10.9734/bmrj/2014/6555